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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in lots of tumors, which leads to chromosome instability, and thus may add to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a significant purpose in selling cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two further splice variants had been described but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind particularly on the focus on antibody and block antibody binding. These peptide ordinarily has the epitope regarded through the antibody.
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This tutorial outlines even more achievable optimization of mobile and immunoassay parameters to make certain the best possible final results are...
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This information outlines further probable optimization of cellular and immunoassay parameters to be sure the very best results are...
Antibodies bound to the blocking peptide no longer bind on the epitope on the focus on protein. By evaluating the staining from click here your blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs for the very conserved cyclin loved ones, whose members are characterised by a dramatic periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit distinct expression and degradation styles which lead into the temporal coordination of every mitotic event. Cyclin E varieties a complex with and capabilities as being a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S phase boundary and it is degraded as cells development through S period.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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